FOIA Would you like email updates of new search results? Molecular weight, molecular weight distribution and molecular size of a native dextran. ; Aberle, T; Burchard, W. (2000). 2010;4(1):117. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Biomaterials. ; Bos, G.W. A single frequency laser is directed to the sample contained in a cuvette. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Microporation techniques for enhanced delivery of therapeutic agents. Alternatives to animal testing: a review. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Viscotek GmbH, Europe. American Journal of Food Science and Technology 2013, 1(3), 60-66. 8580 Macromolecules 34, Notes - NIST NCI CPTC Antibody Characterization Program. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Cite this article. Eng. Journal of Applied Polymer Science, 27, 795-810. Polymolecularity and polydispersity in Molecular weight determinations. ; Wood, P.J. Materiaux 1-5. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Carbohydrate Research, 160, 205-214. Chem. Drug Discov Today Technol. where tr is the column retention time in minutes (2 of 0.9792). Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Macromolecules, 29, 2265-2268. De Belder, A.N. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. 2006;3(3):37385. The effect of branching is best recognized from the molecular weight dependencies of []. Study of temperature influence. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Kasaai, Mohammad R. (2007). Structural and biocompatibility properties of dextran from J. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. 2005;22(4):5505. Carbohydrate Polymers 72, 356-360. (1958). Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. TEWL measurements were performed before and 30min after microporation. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. (2004). H&E stained images were analyzed using Image J software. dextran hydrodynamic radius. Radiofrequency with microneedling. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. 20K. * shows a significant difference between the M.W. IV. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) (1990). Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Arch Dermatol Res. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Webdextran hydrodynamic radius. Masuelli, Martin Alberto. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. II. The Mark-Houwink. dextran hydrodynamic radius eCollection 2018 Feb. Molecules. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. The Mark-Houwink a constant is close to 0.5 in poor solvents. The hydrodynamic radius of 2-NBDG glucose was too small to measure. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Unable to load your collection due to an error, Unable to load your delegates due to an error. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). (2012). Catiker, E.; Guner, A. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Webwhittier union high school district superintendent. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Progress in Polymer Science, 30, 38-70. Carbohydrate Research, 340, 811-826. Charge: Anionic. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. This conjecture found to be correct. Monolayers and Thin Films of Dextran Hydrophobically Modified. Carbohydrate Polymers 88 1440-1444. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Determination of polymer structure by Gel Permeation Chromatography. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. The micropore formed at the SC through to the epidermis, enabling drug delivery. Sung-Wook Choi. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Advances in Physical Chemistry, Vol. hydrodynamic Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. dextran hydrodynamic radius. Biomaterials Research Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. FITC-dextran solution (600L) was added to the donor chamber. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Increased TEWL values are evidence of micropores formation. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. Expert Rev Med Devices. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte (1937). Global hydrodynamic analysis of the molecular flexibility of galactomannans. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Advances in Carbohydrate Analysis, 1, 63-144. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. WebTry out PMC Labs and tell us what you think. Journal of Controlled Release, 119, 5-24. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). The .gov means its official. 2019;26(1):25261. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. 2011;16(2):518. Google Scholar. The solution was measured using an Anton Paar DMA35N densitometer. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. WebR H Hydrodynamic radius [m] The basic DLS setup. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. WebDextran 40 Pharmaceutical Quality. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. 113, 81-86. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Thus, it is a common practice to express the frictional coefficient of rigid structures as. Polysaccharide hydrogels for modified release formulations. Guner, A.; Kibarer, G. (2001). Nanoparticles in magnetic resonance imaging: from simple to dual The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Characterisation of insulin analogues therapeutically available to Experimental and numerical study on the mixed lubrication Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. The micropores of the human cadaver skin were regenerated according to the time. Nair SS. The human and pig skin permeability of FITC-dextran (M.W. (2013). (2007). Small Intestinal Permeability and Gut-Transit Time Determined Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Harding, Stephen E. (1997). Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. All branched polysaccharide types could be described approximately by the hyperbranching theory. Journal of Chemical Education, 80(9), 1036-1038. Macromolecules. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. International Journal of Biological Macromolecules, 42, 133-137. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Cookies policy. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). Environ Toxicol Pharmacol. Advanced Drug Delivery Reviews, 60, 1650-1662. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. (1969). Walkenhorst, S.; Olivier, R. (2003). a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Journal Argentine Chemical Society 100 (2013) 25-34. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Carbohydrate Polymers 99, 199-207. MeSH WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Not for use in diagnostic procedures. 2009;6(4):34354. dextran Optical properties of dextran in solution and in films. Bookshelf Determination of intrinsic viscosities of macromolecules and nanoparticles. Int J Food Sci Nutr. Expert Opin Drug Deliv. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. PubMedGoogle Scholar. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Normally, the skin loses very little water loss whereas damaged skin loses more [22]. J Appl Toxicol. Biophysical Journal, 87, 4259-4270. 2. Article 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. ; Ross-Murphy, S.B. For data requests, please contact the authors. Chem. Herwadkar A, Banga AK. Drug Deliv. ; Hogan, M.P. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. Solution properties of branched dextran. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Nanoparticles in magnetic resonance imaging: from simple to dual The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Harding, S. E., & Simpkin, N. J. Dextran produced from Weissella cibaria JAG8 was purified and characterized. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Pharm Res. De Belder, A. N. (2001). Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. The term f/f0 is sometimes denoted as P, Perrin constant. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), (2003). The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Analytical Chemistry. Epub 2021 Feb 9. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<).